Chemical & Biomolecular Engineering

Top 20 Doctoral Program—National Research Council

Location

Dept. of Chemical & Biomolecular Engineering
University of Houston
S222 Engineering Bldg 1,
Houston, TX 77204-4004
Phone: 713-743-4300
Campus Map

Faculty

Dr. Navin Varadarajan

Assistant Professor of Chemical and Biomolecular Engineering

Office Location: S228

Tel: (713) 743-1691 | Fax: (713) 743-4323

Email: nvaradarajan [at] uh [dot] edu

Website: http://singlecell.chee.uh.edu

Education

  • B.S. Chemistry, University of Madras (1998)
  • M.S. Organic Chemistry, India Institute of Science (2001
  • Ph.D. Chemistry, University of Texas at Austin (2006)

Research Interests

Our lab is developing high-throughput screens designed to characterize a wide range of biological functions ranging from the properties of proteins in single cells to antigen mediated cellular cytotoxicity. The development of these assays should serve as versatile platforms for the systemic investigation of B cells and antibodies in autoimmune diseases; characterizing T cell responses  in cancer and engineering therapeutic enzymes/antibodies.

Awards and Honors

  • 2009-Present Member, American Institute of Chemical Engineers
  • 2006-Present Member, American Chemical Society
  • 1998-2001 Indian Institute of Science, Integrated PhD Fellowship

Selected Publications

  1. Dekosky BJ, Ippolito GC, Deschner RP, Lavinder JJ, Wine Y, Rawlings BM, Varadarajan N, Giesecke C, Dörner T, Andrews SF, Wilson PC, Hunicke-Smith SP, Willson CG, Ellington AD, Georgiou G,

    "High-throughput sequencing of the paired human immunoglobulin heavy and light chain repertoire," Nat Biotech Jan 20; 31(2):166-. doi: 10.1038/nbt.2492

    , 2013
  2. Liadi I, Roszik J, Romain G, Cooper LJN, Varadarajan N,

    "Quantitative High-Throughput Single-Cell Cytotoxicity Assay for T cells," J Vis. Exp. (72) doi: 10.3791/50058

    , 2013
  3. Sendra VG, Lie A, Romain G, Agarwal SK, Varadarajan N,

    "Detection and isolation of auto-reactive human antibodies from primary B cells," Methods 10.1016/j.ymeth.2013.06.018

    , 2013
  4. Ramesh B, Sendra VG, Cirino PC, Varadarajan N,

    "Single-cell characterization of autotransporter mediated Escherichia coli surface display of disulfide-bond containing proteins," J Biol Chem Nov 9;287(46):38580-9. doi: 10.1074/jbc

    , 2012
  5. Varadarajan N, Julg B, Yamanaka YJ, Chen H, Ogunniyi AO, McAndrew E, Porter LC, Piechocka-Trocha A, Hill BJ, Douek DC, Pereyra F, Walker BD, Love JC,

    “A high-throughput single-cell analysis of human CD8+ T cell functions reveals discordance for cytokine secretion and cytolysis,” J Clin Invest, 121(11):4322-31

    , 2011
  6. Varadarajan N, Cantor J, Georgiou G and Iverson BL,

    “Construction and flow-cytometric screening of targeted enzyme libraries,” Nature Protocols, 4(6):893-901

    , 2009
  7. Varadarajan N, Pogson M, Georgiou G and Iverson BL,

    “A three color multi-substrate flow-cytometric assay to engineer protease specificity for nitro-tyrosine,” J. Am. Chem. Soc., 131(50):18186-18190. DOI: 10.1021/ja907803k

    , 2009
  8. Varadarajan N, Georgiou G, Iverson BL,

    “Engineered Proteases that Cleave Specifically after Sulfalted Tyrosine for the Detection of Post-Translationally Modified Peptides,” Angew Chem Intl Ed, 47(41):7861

    , 2008
  9. Varadarajan N, Rodriguez S, Hwang BY, Georgiou G, Iverson BL,

    “Engineering a Family of Highly Active and Selective Endopeptidases with Programmed Substrate Specificities,” Nature Chemical Biology, 4(5):290-4

    , 2008
  10. Hwang BY, Varadarajan N, Li H, Rodriguez S, Iverson BL, Georgiou G,

    “Substrate Specificity of the Escherichia coli Outer Membrane Protease OmpP,” J. Bacteriology, 189: 522-30

    , 2007
  11. Varadarajan N, Gam J, Olsen MJ, Georgiou G, Iverson BL,

    “Engineering of protease variants exhibiting high catalytic activity and exquisite substrate selectivity,” Proc Natl Acad Sci U S A, 102:6855-60

    , 2005