Faculty

Dr. Katerina Kourentzi
Dr. Katerina Kourentzi

Research Associate Professor of Chemical and Biomolecular Engineering

Office Location: S185A, Engineering Building 1
Phone: 832-842-6301   |   Fax: 713-743-4323
Email: edkourentzi [at] uh [dot] edu

Education: 

Diploma Chemical Engineering, National Technical University, Athens, Greece (1996)
Ph.D. Chemical Engineering, University of Houston, Houston (2002)

Courses: 

Chemical Engineering Thermodynamics II_CHEE 3333 (Fall 2008, Fall 2009, Spring 2009)

Engineering Computing_ CHEE 1331 (Spring 2014, Fall 2015, Spring 2016)

Research Interests: 

Development of novel diagnostic technologies (esp. lateral-flow tests) for the early detection of disease.

Awards & Honors: 

2007: Roche Younger Investigator Award, Affinity 2007 Conference, International Society for Molecular Recognition

2000: Student Research Poster Award, Sigma Xi, University of Houston Chapter

1999: Hellenic Professional Society of Texas Scholarship for Excellence in Academic Achievement

Professional Activities: 

2011 - Present: Editorial Board, PLOS ONE

1998 - Present: Member, American Chemical Society (ACS); Division of Biochemical Technology Peterson Award committee co-chair (2003-2005)

Selected Publications

  1. Chavan D, Chen H, Crum M, Vu B, Safari M, Smith M , Vekilov P, Conrad JC, Kourentzi K, Willson RC ,

    Neutral DNA-avidin nanoparticles as ultrasensitive reporters in immuno-PCR. Analyst, 145(14):4942-4949

    , 2020
  2. Danthanarayana AN, Finley E, Vu B, Kourentzi K, Willson RC, Brgoch J,

    A multicolor multiplex lateral flow assay for high-sensitivity analyte detection using persistent luminescent nanophosphors. Analytical Methods, 12, 272-280

    , 2020
  3. Kourentzi K, Crum M, Patil U, Prebisch A, Chavan D, Vu B, Zeng Z, Litvinov D, Zu Y, Willson RC,

    Recombinant expression, characterization, and quantification in human cancer cell lines of the Anaplastic Large-Cell Lymphoma-characteristic NPM-ALK fusion protein. Scientific Reports, 10(1): 5078

    , 2020
  4. Goux HJ, Raja B, Kourentzi K, Trabuco JRC, Vu BV, Paterson AS, Kirkpatrick A, Townsend B, Lee M, Truong VTT, Pedroza C, Willson RC,

    Evaluation of a nanophosphor lateral-flow assay for self-testing for herpes simplex virus type 2 seropositivity.
    PLoS One, 14(12): e0225365

    , 2019
  5. Khodadadi M, Chang L, Trabuco JRC, Vu BV, Kourentzi K, Willson RC, Litvinov D,

    PCB-Based Magnetometer as a Platform for Quantification of Lateral-Flow Assays. Sensors (Basel). 19(24) pii: E5433

    , 2019
  6. Chen H, Crum M, Chavan D, Vu B, Kourentzi K, Willson RC,

    Nanoparticle-Based Proximity Ligation Assay for Ultrasensitive, Quantitative Detection of Protein Biomarkers. ACS Appl Mater Interfaces, 10(38): 31845-31849

    , 2018
  7. Goux HJ, Chavan D, Crum M, Kourentzi K, Willson RC,

    Akkermansia muciniphila as a Model Case for the Development of an Improved Quantitative RPA Microbiome Assay. Frontiers in Cellular & Infection Microbiology, 8: 237

    , 2018
  8. Goux HJ, Raja B, Kourentzi K, Trabuco JRC, Vu BV, Paterson AS, Kirkpatrick A, Townsend B, Lee M, Truong VTT, Pedroza C, Willson RC ,

    Nanoparticle-Based Proximity Ligation Assay for Ultrasensitive, Quantitative Detection of Protein Biomarkers.
    ACS Appl Mater Interfaces, 10(38): 31845-31849

    , 2018
  9. Jacinto MJ, Trabuco JRC, Vu BV, Garvey G, Khodadady M, Azevedo AM, Aires-Barros MR, Chang L, Kourentzi K, Litvinov D, Willson RC,

    Enhancement of lateral flow assay performance by electromagnetic relocation of reporter particles. PLOS One, 13:e0186782

    , 2018
  10. Kim J, Vu B, Kourentzi K, Willson RC, Conrad JC,

    Increasing Binding Efficiency via Reporter Shape and Flux in a Viral Nanoparticle Lateral-Flow Assay. ACS Appl Mater Interfaces, 9: 6878-84

    , 2017
  11. Kisley L, Patil U, Dhamane S, Kourentzi K, Tauzin LJ, Willson RC, Landes CF,

    Competitive multicomponent anion exchange adsorption of proteins at the single molecule level. Analyst, 142 3127-3131

    , 2017
  12. Liang Y, Chang L, Qiu W, Kolhatkar AG, Vu B, Kourentzi K, Lee TR, Zu Y, Willson R, Litvinov D. ,

    Ultrasensitive Magnetic Nanoparticle Detector for Biosensor Applications. Sensors, 17, pii: E1296

    , 2017
  13. Qiu W, Chang L, Linag YC, Litvinoc J, Guo J, Chen YT, Vu B, Kourentzi K, Xu S, Lee RT, Zu Y, Willson RC, Litvinov D. ,

    Spin-Valve based magnetoresistive nanoparticle detector for applications in biosensing. Sensors and Actuators A: Physical, 265: 174-80

    , 2017
  14. Raja B, Goux HJ, Marapadaga A, Rajagopalan S, Kourentzi K, Willson RC.,

    Development of a panel of recombinase polymerase amplification assays for detection of common bacterial urinary tract infection pathogens. J Appl Microbiol, 123: 544-555

    , 2017
  15. Chen H, Hagström AE, Kim J, Garvey G, Paterson A, Ruiz-Ruiz F, Raja B, Strych U, Rito-Palomares M, Kourentzi K, Conrad JC, Atmar RL, Willson RC,

    Flotation Immunoassay: Masking the Signal from Free Reporters in Sandwich Immunoassays. Scientific Reports, 6: 2497

    , 2016
  16. Kim J, Poling-Skutvik R, Trabuco JR, Kourentzi K, Willson RC, Conrad JC,

    Orientational binding modes of reporters in a viral-nanoparticle lateral flow assay. Analyst, 142: 55-64

    , 2016
  17. Kisley L, Poongavanam MV, Kourentzi K, Willson RC, Landes CF,

    pH-dependence of single-protein adsorption and diffusion at a liquid chromatographic interface. J Sep Sci, 39: 682-8

    , 2016
  18. Kolhatkar AG, Jamison AC, Nekrashevich I, Kourentzi K, Litvinov D, Brazdeikis A, Willson RC, Lee RT,

    Enzymatic conversion of magnetic nanoparticles to a non-magnetic precipitate: a new approach to magnetic sensing. Analyst, 141: 5246-5251

    , 2016
  19. Poongavanam MV, Kisley L, Kourentzi K, Landes C, Willson RC,

    Ensemble and Single-Molecule Biophysical Characterization of D17.4 DNA Aptamer-IgE Interactions. Biochim Biophys Acta, 864: 154-64

    , 2016
  20. Raja B, Pascente C, Knoop J, Shakarisaz D, Sherlock T, Kemper S, Kourentzi K, Renzi R F, Hatch AV, Olano J, Peng BH, Ruchhoeft P, Willson RC,

    An embedded microretroreflector-based microfluidic immunoassay platform. Lab on a chip, 6: 1625-35

    , 2016
  21. Xie M, Wang S, Singh A, Cooksey TJ, Marquez MD, Bhattarai A, Kourentzi K, Robertson ML,

    Fluorophore exchange kinetics in block copolymer micelles with varying solvent-fluorophore and solvent-polymer interactions. Soft Matter, 12: 6196-205

    , 2016
  22. Adhikari M, Strych U, Kim J, Goux H, Dhamane S, Poongavanam MV, Hagstrom AEV, Kourentzi K, Conrad JC, and RC Willson,

    Aptamer-phage reporters for ultrasensitive lateral flow assays. Anal Chem. 87(23): 11660–11665

    , 2015
  23. Hagström AE, Garvey G, Paterson AS, Dhamane S, Adhikari M, Estes MK, Strych U, Kourentzi K, Atmar RL, Willson RC,

    Sensitive detection of norovirus using phage nanoparticle reporters in lateral-flow assay. PLoS One. 10(5): e0126571

    , 2015
  24. Kolhatkar AG, Dannongoda C, Kourentzi K, Jamison AC, Nekrashevich I, Kar A, Cacao E, Strych U, Rusakova I, Martirosyan KS, Litvinov D, Lee TR, Willson RC,

    Enzymatic synthesis of magnetic nanoparticles. Int J Mol Sci, 16:7535-50

    , 2015
  25. Dhamane, S, F Ruiz-Ruiz, WH Chen, K Kourentzi, J Benavides, M Rito-Palomares, and RC Willson,

    Spermine Sepharose as a clustered-charge anion exchange adsorbent. Journal of Chromatography A, 1324: p. 135-140

    , 2014
  26. Garvey G, Shakarisaz D, Ruiz-Ruiz F, Hagström AE, Raja B, Pascente C, Kar A, Kourentzi K, Rito-Palomares M, Ruchhoeft P, Willson RC. ,

    Microretroreflector-sedimentation immunoassays for pathogen detection. Anal Chem, 86: 9029-35

    , 2014
  27. Kisley, L, JX Chen, AP Mansur, B Shuang, K Kourentzi, MV Poongavanam, WH Chen, S Dhamane, RC Willson, and CF Landes,

    Unified superresolution experiments and stochastic theory provide mechanistic insight into protein ion-exchange adsorptive separations. Proceedings of the National Academy of Sciences of the United States of America, 111(6): p. 2075-2080

    , 2014
  28. Kisley, L, JX Chen, AP Mansur, S Dominguez-Medina, E Kulla, MK Kang, B Shuang, K Kourentzi, MV Poongavanam, S Dhamane, RC Willson, and CF Landes,

    High ionic strength narrows the population of sites participating in protein ion-exchange adsorption: A single-molecule study. Journal of Chromatography A, 1343: p. 135-142

    , 2014
  29. Kourentzi, K and RC Willson,

    Nanobiotechnology for Medical Diagnostics. Revista Mexicana De Ingenieria Quimica, 13(1): p. 9-18

    , 2014
  30. Litvinov, J, AE Hagstrom, Y Lopez, M Adhikari, K Kourentzi, U Strych, FA Monzon, W Foster, PT Cagle, and RC Willson,

    Ultrasensitive immuno-detection using viral nanoparticles with modular assembly using genetically-directed biotinylation. Biotechnol Lett, 36(9): p. 1863-8

    , 2014
  31. Paterson AS, Raja B, Garvey G, Kolhatkar A, Hagstrom AE, Kourentzi K, Lee TR, Willson RC,

    Persistent Luminescence Strontium Aluminate Nanoparticles as Reporters in Lateral Flow Assays. Anal Chem. p.9481-9499

    , 2014
  32. Wang, YA, A Kar, A Paterson, K Kourentzi, H Le, P Ruchhoeft, R Willson, and JM Bao,

    Transmissive Nanohole Arrays for Massively-Parallel Optical Biosensing. Acs Photonics, 1(3): p. 241-245

    , 2014
  33. Adhikari, M, S Dhamane, AEV Hagstrom, G Garvey, WH Chen, K Kourentzi, U Strych, and RC Willson,

    Functionalized viral nanoparticles as ultrasensitive reporters in lateral-flow assays. Analyst, 138(19): p. 5584-5587

    , 2013
  34. Cacao, E, T Sherlock, A Nasrullah, S Kemper, J Knoop, K Kourentzi, P Ruchhoeft, GE Stein, RL Atmar, and RC Willson,

    Helium beam shadowing for high spatial resolution patterning of antibodies on microstructured diagnostic surfaces. Biointerphases, 8

    , 2013
  35. Cacao, EE, A Nasrullah, T Sherlock, S Kemper, K Kourentzi, P Ruchhoeft, GE Stein, and RC Willson,

    High-Resolution, High-Throughput, Positive-Tone Patterning of Poly(ethylene glycol) by Helium Beam Exposure through Stencil Masks. Plos One, 8(5)

    , 2013
  36. Jaeger, IS, I Kretzschmar, J Korner, AA Weiser, CC Mahrenholz, A Potty, K Kourentzi, RC Willson, R Volkmer, and R Preissner,

    Mapping discontinuous protein-binding sites via structure-based peptide libraries: combining in silico and in vitro approaches. Journal of Molecular Recognition, 26(1): p. 23-31

    , 2013
  37. Kanakaraj, I, WH Chen, M Poongavanam, S Dhamane, LJ Stagg, JE Ladbury, K Kourentzi, U Strych, and RC Willson,

    Biophysical characterization of VEGF-aHt DNA aptamer interactions. International Journal of Biological Macromolecules, 57: p. 69-75

    , 2013
  38. Wang, S, T Sherlock, B Salazar, N Sudheendran, RK Manapuram, K Kourentzi, P Ruchhoeft, RC Willson, and KV Larin,

    Detection and Monitoring of Microparticles Under Skin by Optical Coherence Tomography as an Approach to Continuous Glucose Sensing Using Implanted Retroreflectors. Ieee Sensors Journal, 13(11): p. 4534-4541

    , 2013
  39. Daniels, CR, L Kisley, H Kim, WH Chen, MV Poongavanam, C Reznik, K Kourentzi, RC Willson, and CF Landes,

    Fluorescence correlation spectroscopy study of protein transport and dynamic interactions with clustered-charge peptide adsorbents. Journal of Molecular Recognition, 25(8): p. 435-442

    , 2012
  40. Chen, WH, JY Fu, K Kourentzi, and RC Willson,

    Nucleic acid affinity of clustered-charge anion exchange adsorbents: Effects of ionic strength and ligand density. Journal of Chromatography A, 1218(2): p. 258-262

    , 2011
  41. Daniels, CR, C Reznik, R Kilmer, MJ Felipe, MCR Tria, K Kourentzi, WH Chen, RC Advincula, RC Willson, and CF Landes,

    Permeability of anti-fouling PEGylated surfaces probed by fluorescence correlation spectroscopy. Colloids and Surfaces B-Biointerfaces, 88(1): p. 31-38

    , 2011
  42. Potty, ASR, K Kourentzi, H Fang, P Schuck, and RC Willson,

    Biophysical characterization of DNA and RNA aptamer interactions with hen egg lysozyme. International Journal of Biological Macromolecules, 48(3): p. 392-397

    , 2011
  43. Sherlock, T, A Nasrullah, J Litvinov, E Cacao, J Knoop, S Kemper, K Kourentzi, A Kar, P Ruchhoeft, and R Willson,

    Suspended, micron-scale corner cube retroreflectors as ultra-bright optical labels. Journal of Vacuum Science & Technology B, 29(6)

    , 2011
  44. Ivers, SN, SA Baranov, T Sherlock, K Kourentzi, P Ruchhoeft, R Willson, and KV Larin,

    Depth-resolved imaging and detection of micro-retroreflectors within biological tissue using Optical Coherence Tomography. Biomedical Optics Express, 1(2): p. 367-377

    , 2010
  45. Mohan, S, K Kourentzi, KA Schick, C Uehara, CA Lipschultz, M Acchione, ME DeSantis, SJ Smith-Gill, and RC Willson,

    Association Energetics of Cross-Reactive and Specific Antibodies. Biochemistry, 48(6): p. 1390-1398

    , 2009
  46. Potty, ASR, K Kourentzi, H Fang, GW Jackson, X Zhang, GB Legge, and RC Willson,

    Biophysical Characterization of DNA Aptamer Interactions with Vascular Endothelial Growth Factor. Biopolymers, 91(2): p. 145-156

    , 2009
  47. Zhang, X, ASR Potty, GW Jackson, V Stepanov, A Tang, Y Liu, K Kourentzi, U Strych, GE Fox, and RCW Willson,

    Engineered 5S Ribosomal RNAs Displaying Aptamers Recognizing Vascular Endothelial Growth Factor and Malachite Green. Journal of Molecular Recognition, 22(2): p. 154-161

    , 2009
  48. Kourentzi, K, M Srinivasan, SJ Smith-Gill, and RC Willson,

    Conformational flexibility and kinetic complexity in antibody-antigen interaction. Journal of Molecular Recognition, 21(2): p. 114-121

    , 2008
  49. Peterson, KJ, JD Sadowsky, EA Scheef, S Pal, KD Kourentzi, RC Willson, EH Bresnick, N Sheibani, and SH Gellman,

    A fluorescence polarization assay for identifying ligands that bind to vascular endothelial growth factor. Analytical Biochemistry, 378(1): p. 8-14

    , 2008
  50. Taylor, JN, Q Darugar, K Kourentzi, RC Willson, and CF Landes,

    Dynamics of an anti-VEGF DNA aptamer: A single-molecule study. Biochemical and Biophysical Research Communications, 373(2): p. 213-218

    , 2008
  51. Larios-Sanz, M, KD Kourentzi, D Warmflash, J Jones, DL Pierson, RC Willson, and GE Fox,

    16S rRNA beacons for bacterial monitoring during human space missions. Aviation Space and Environmental Medicine, 78(4): p. A43-A47

    , 2007
  52. Kourentzi, KD, GE Fox, and RC Willson,

    Hybridization-responsive fluorescent DNA probes containing the adenine analog 2-aminopurine. Analytical Biochemistry, 322(1): p. 124-126

    , 2003
  53. Kourentzi, KD, GE Fox, and RC Willson,

    Microbial identification by immunohybridization assay of artificial RNA labels. Journal of Microbiological Methods, 49(3): p. 301-306

    , 2002
  54. Kourentzi, KD, GE Fox, and RC Willson,

    Microbial detection with low molecular weight RNA. Current Microbiology, 43(6): p. 444-447

    , 2001
  55. Hatzinikolaou, DG, E Kourentzi, H Stamatis, P Christakopoulos, FN Kolisis, D Kekos, and BJ Macris,

    A novel lipolytic activity of Rhodotorula glutinis cells: Production, partial characterization and application in the synthesis of esters. Journal of Bioscience and Bioengineering, 88(1): p. 53-56

    , 1999
  56. Christakopoulos, P, E Kourentzi, DG Hatzinikolaou, M Claeyssens, D Kekos, and BJ Macris,

    Enhancement of pH-stability of a low molecular mass endoglucanase from Fusarium oxysporum by protein pegylation. Carbohydrate Research, 314(1-2): p. 95-99

    , 1998
  57. Macris, JB, E Kourentzi, and DG Hatzinikolaou,

    Studies on localization and regulation of lipase production by Aspergillus niger. Process Biochemistry, 31(8): p. 807-812

    , 1996